ABI5 overexpression has been shown to resemble ABI3 overexpression in that it confers hypersensitivity to ABA inhibition of root growth and promotes slightly

ZFP3 overexpression in the Col-0, abi2-1, and abi4-. 101 backgrounds reduced hypocotyl lengths by. 50% to 60%, while in abi5-1/ZFP3ox seedlings, hy-.

Biochemical interaction assays demonstrated that ABI5 physically interacted with the RIBOSOMAL S6 KINASE2 (S6K2) protein in plant cell. It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present experiment, we showed that 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of the loss-of-function mutant abi5-1, but promotes seed germination of the ABI5-overexpression transgenic line, and that ABI5 affects reactive oxygen species (ROS) homeostasis. ABI5 overexpression rescued the growth arrest phenotype at low CO 2. Taken together, our results demonstrate that PPC2 and ABI5 are key regulators of plant acclimation to low CO 2, and positively contribute to carbon fixation and metabolism in C 3 plants. Pharmacological assay showed that abi5-1 mutant was insensitive to TOR inhibitor AZD8055, whereas AtABI5 overexpression lines were hypersensitive to AZD8055 in Arabidopsis. Biochemical interaction assays demonstrated that ABI5 physically interacted with the RIBOSOMAL S6 KINASE2 (S6K2) protein in plant cell.

50% to 60%, while in abi5-1/ZFP3ox seedlings, hy-. 30 Nov 2019 ABI5 emerges as a critical ABA-signaling component in the regulation of the plant Overexpression of another bZIP TF, ABI5, upregulates FLC. av E Henriksson · 2004 · Citerat av 6 — insensitive loci, ABI3, ABI4 and ABI5, encode transcription factors of the part of the overexpression phenotypes reflects the function of these genes in wild-type. The Arabidopsis DELAY OF GERMINATION 1 gene affects ABSCISIC ACID INSENSITIVE 5 (ABI5) expression and genetically interacts with ABI3 during specific phenotype, but overexpression analysis suggested a role for Oshox4 in gene activity is down-regulated in the abil-1, abi3-1 and abi5-1 mutant lines, Detta bevisar det faktum att komplexet av LtWRKY21, VP1 och ABI5 TaWRKY2 and TaWRKY19 overexpression in Arabidopsis led to more efficient salt, Planttillväxt; Protein Overexpression and Purification; ytterligare information 23, 24, 25, 26 och transkriptionsfaktorer (TFs) ) såsom ABI5 eller AREB1 4 . It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present study, we showed that inhibition of the catalase activity with 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of Col-0, abi5 mutants and ABI5-overexpression transgenic lines. Loss-of-function in ABI5 (abi5) promotes juvenile-to-adult transition, whereas overexpression of ABI5 delays this transition in short days. Genetic analyses indicated that the effect of mir159ab on vegetative phase change is ABI5 dependent.

Furthermore, we showed that ABI5 directly binds to the CAT1promoter and activates CAT1expression. Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germi- nation. Bar, 1 cm.

Loss‐of‐function in ABI5 (abi5) promotes juvenile‐to‐adult transition, whereas overexpression of ABI5 delays this transition in short days. Genetic analyses indicated that the effect of mir159ab on vegetative phase change is ABI5 dependent.

Overexpression of RAV1 repressed ABI3, ABI4, and ABI5 expression, and RAV1 bound to the ABI3, ABI4, and ABI5 promoters in vitro and in vivo, indicating that RAV1 directly down-regulates the expression of ABI3, ABI4, and ABI5. Conversely, overexpression of ABI5 enhanced light‐mediated hypocotyl inhibition in seedlings (Chen et al., 2008). Intriguingly, the regulatory interactions among light signalling factors while they modulate ABA signalling can sometimes be different from their primary interactions during light‐related developmental processes.

Indeed, stomatal aperture was significantly reduced by ABI5 overexpression in the absence or presence of ABA under monochromatic light conditions. Overall, we present a regulatory loop in which two light signalling proteins repress ABA signalling to sustain gas …

2002; Söderman et al.

ABI5 production is enhanced by stress including high salt and drought. This protects plants from drought. (a) ABI5‐HA fusion protein was detected by the anti‐HA antibody in ABI5 overexpression transgenic plants. (b) Analysis of the expression level of AtIPT8 by semi‐quantitative RT‐PCR.
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In the present experiment, we showed that 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of the loss-of-function mutant abi5-1, but promotes seed germination of the ABI5-overexpression transgenic line, and that ABI5 affects reactive oxygen species (ROS) homeostasis. ABI5 overexpression rescued the growth arrest phenotype at low CO 2. Taken together, our results demonstrate that PPC2 and ABI5 are key regulators of plant acclimation to low CO 2, and positively contribute to carbon fixation and metabolism in C 3 plants. Pharmacological assay showed that abi5-1 mutant was insensitive to TOR inhibitor AZD8055, whereas AtABI5 overexpression lines were hypersensitive to AZD8055 in Arabidopsis.

ABI5 was previously reported as the target protein of AFP2 during seed germination (Lopez-Molina et al., 2003), and overexpression of ABI5 activates the flowering negative factor FLC at the transcriptional level to repress flowering (Wang et al., 2013b). Thus, we compared the transcription of FLC in Col, afp2, and AFP2-ox under LD conditions. Loss-of-function in ABI5 (abi5) promotes juvenile-to-adult transition, whereas overexpression of ABI5 delays this transition in short days.
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2020-12-01 · FOF2 inhibits ABA-mediated seed germination partially by repressing ABI3 and ABI5. • Overexpression of FOF2 enhances plant drought tolerance. • FOF2 promotes ABA-induced stomatal closure and ABA biosynthesis under drought stress.

Expression of ABI5 is strongly induced by exogenous ABA (Lopez‐Molina et al., 2001). Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ-accumulating (JAZ-ΔJas) plants. Together, our results reveal a previously uncharacterized signaling module in which JAZ repressors of the JA pathway regulate the ABA-responsive ABI3 and ABI5 transcription factors to integrate JA and ABA signals during seed germination and post-germinative growth. ABI5 overexpression also results in high sensitivity to glucose and anthocyanin accumulation in response to sugar stress (Finkelstein et al., 2002). Analyses of transcript accumulation in abi5 mutants suggest that, similar to ABI3, ABI5 has both activator and repressor functions that may have either synergistic or antagonistic effects on gene expression, depending on the target gene.